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  1. In addition, these publications lack sufficient data for a detailed analysis for Sprague-Dawley rats stratifying by sex, and use the mean and standard deviations for establishing reference intervals, which are not the CLSI and ASVCP recommendations. Conclusions Understanding the significant discrepancies in hematologic and biochemical analytes between male and female Sprague-Dawley rats provides important insight into physiological effects in test rats. Males had higher hemoglobin, hematocrit, red blood cell count, red cell distribution width, mean corpuscular volume, mean corpuscular hemoglobin, white blood cell count, neutrophils, lymphocytes, monocytes, percentage of neutrophils, percentage of monocytes, alanine aminotransferase, aspartate aminotransferase, and triglycerides compared to females.

  2. Within-day precision was calculated based on testing samples from healthy individual 20 times within the same day while between-day precision was assessed by measuring controls over consecutive days from May to December

  3. Clinical and Laboratory Standards Institute CLSI document CA3 is a standard for defining, establishing and verifying reference intervals in the clinical laboratory [ 1 ]. At the beginning of treatment, the rats were around 5 weeks of age. Drinking water and full nutritional granular feed were supplied ad libitum to the animals.

  4. The animals were kept at room temperature for 15 to 25min after the administration to induce anesthesia.

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